Adult mesenchymal stem cells (MSCs) are being investigated further for their use in stem cell therapies. However, as they are\nfound in very low numbers in adult tissue, expansion in vitro is required to produce desired MSC numbers for clinical application.\nThe need for effective cell-based therapies is increasing due to a rise in the ageing population, increasing the prevalence of\nmusculoskeletal disorders. This review investigates how factors, age and gender of donor, as well as seeding density can affect MSC\nexpansion. Age and gender of donor have received mixed results from studies, whereas seeding density studies have produced\nconsistent results for numerous MSC sources, favouring lower seeding densities. Further research is required to reduce the risk of\ninfection, loss of cell characterisation in cell culture, and making cell-based therapies more cost effective through creating rapid\nexpansion of MSCs regardless of patient factors.
Loading....